Koichiro Tamura, Daniel Peterson, Nicholas Peterson, Glen Stecher, Masatoshi Nei, and Sudhir Kumar (2011) MEGA5: Molecular Evolutionary Genetics Analysis using Likelihood, Distance, and Parsimony methods. Molecular Biology and Evolution.
(to be submitted)
Kumar S, Dudley J, Nei M & Tamura K (2008) MEGA: A biologist-centric software for evolutionary analysis of DNA and protein sequences. Briefings in Bioinformatics 9: 299-306.
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Tamura K, Dudley J, Nei M & Kumar S (2007) MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Molecular Biology and Evolution 24: 1596-1599.
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Bugs Fixed in Mega 4.0

Main MEGA Bugs

  • MEGA has problems reading the NEXUS/PAUP format file smoothly
  • MEGA3 stops responding or crashes while performing Maximum Parsimony analyses with bootstrap.
  • When performing the MP method, more than one tree with different topologies can be obtained. All trees display the same topology when exported in Newick format, although this is not the case when viewed in Tree Explorer.
  • Division by zero error appears in calculation of TajimaD.
  • MEGA3 crashes when performing ClustalW in larger multiple alignments.
  • The "selection tests" menu doesn't update when genes/domains are marked coding or uncoding.
  • Broken bug report link on MEGA 3 FAQ page.
  • The release build still contains expiration date.
  • Error reading Newick tree files containing bootstrap values.
  • Some bootstrap consensus trees remain stuck in "topology only" mode when recovered from *.mts tree session files.
  • Rate not updated after changing the calibration point and pressing the OK button in Calibrate-Clock.

Alignment Editor

  • ClustalW access violation when aligning large data sets.
  • Standard genetic code table always loaded by Alignment Explorer regardless of user's code table selection.
  • Fail to do Blast search from MEGA due to NCBI's changes to Blast interface (Fixed in MEGA#4022).

Data Explorer

  • Failed to export non-interleaved format sequence data.

File Converter

  • File converter appends data to existing files instead of overwriting

Tree Explorer

  • The cutoff value for generating a bootstrap consensus NJ (ME, MP) tree does not work.
  • Division by zero error occurs when changing tree explorer options.
  • Tree explorer loses user-specified ordering during compress/decompress.
  • Compression state of tree nodes not recovered from tree session.
  • Incorrect drawing of radiation trees recovered from some *.mts tree session files.
  • Unable to display bootstrap consensus tree in radiation format.
  • Tree Explorer initializes in minimized state.
  • Fail to display radial tree after branch collapse. (Fixed in MEGA#4022)