MantisBT - MEGA
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IDProjectCategoryView StatusDate SubmittedLast Update
0001023MEGACalculation Errorpublic2018-12-04 02:232018-12-05 10:25
Reporterguest 
Assigned Togstecher 
PrioritynormalSeverityminorReproducibilityhave not tried
StatusresolvedResolutionno change required 
PlatformPCOSWindowsOS Version10
Product VersionMEGA 11 (Graphical Interface version) 
Target VersionFixed in Version 
First NameWeifeng
Last NameShi
Emailshiwf@ioz.ac.cn
Confirm Emailshiwf@ioz.ac.cn
Summary0001023: inconsistent distance estimates
DescriptionDear Professor Kumar,
I estimated within group mean distance for a protein alignment using Mega 5 and Mega X (version 10.0.5), respectively. However, I obtained different estimates (huge difference). When I estimated between group mean distance, I found the same problem. I don't know why different versions give different estimates and which version is more reliable.
Hope to hear from you soon. Thank you for your help.
All the best, Weifeng
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Issue History
Date ModifiedUsernameFieldChange
2018-12-04 02:23guestNew Issue
2018-12-04 08:09gstecherAssigned To => gstecher
2018-12-04 08:09gstecherStatusnew => assigned
2018-12-04 08:10gstecherNote Deleted: 0001046
2018-12-04 08:10gstecherNote Added: 0004150
2018-12-05 10:25gstecherNote Added: 0004152
2018-12-05 10:25gstecherStatusassigned => resolved
2018-12-05 10:25gstecherResolutionopen => no change required
2018-12-05 10:25gstecherNote Added: 0004153

Notes
(0004150)
gstecher   
2018-12-04 08:10   
Hi Weifeng,

I am writing in response to the bug report you recently submitted regarding the MEGA Software. I am not able to reproduce any difference in calculating within or between group mean distances when using MEGA5 and MEGAX. No matter what data and settings I use, I always get exactly the same results. Can you send me the data used as well as screen shots of the Analysis Preferences used so that I can reproduce the issue?

--
Best regards,
Glen Stecher
Institute for Genomics and Evolutionary Medicine
igem.temple.edu
(0004152)
gstecher   
2018-12-05 10:25   
Hi Weifeng,

I get the same results between MEGA5 and MEGAX with your data. Please make sure that you are using the same options and data subset between the different versions of MEGA.
On 12/4/2018 5:41 PM, Weifeng Shi wrote:
> Dear Glen,
> Glad to hear from you. I have attached my dataset and grouping file, as well as the output. Please test them and get back me the reason leading to the difference.
> Thanks a million for your help.
> All the best, Weifeng
>
> Weifeng Shi
(0004153)
gstecher   
2018-12-05 10:25   
it is a user error