MantisBT - MEGA
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0000103MEGACalculation Errorpublic2016-03-23 13:332016-03-23 13:33
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van Hemert
f.j.vanhemert@amc.uva.nl
f.j.vanhemert@amc.uva.nl
0000103: Frameshift in nts composition calculation
I use MEGAv6 presently and almost have used any MEGA version fruitfully.
I noticed two things:
1. Calculation of the nucleotide composition of a CODING sequence (f.i. starting with AUG) generated frame-shifted data per codon position; see the attached file, of which the MEGA data has been copied directly from the MEGA output spreadsheet.
2. Frame translation is possible in various MEGA screens. I noticed that in one of the options (cannot remember which) introduction of a single gap just upstream of AUG is needed for proper frame translation.
Try f.i. the sequence copied from the attached spreadsheet.
The two bugs may be related.
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? chimp_T1.fasta (794,699) 1969-12-31 17:33
https://megasoftware.net/mantis_bt/
xlsx HKUSpikeNucCompCodons.xlsx (122,788) 2016-03-23 13:33
https://megasoftware.net/mantis_bt/
Issue History
2016-03-23 13:33guestNew Issue
2016-03-23 13:33guestFile Added: HKUSpikeNucCompCodons.xlsx

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