MantisBT - MEGA
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IDProjectCategoryView StatusDate SubmittedLast Update
0001239MEGAAlignment Explorerpublic2019-05-21 03:102019-05-21 08:58
Reporterguest 
Assigned Togstecher 
PrioritynormalSeverityminorReproducibilityhave not tried
StatusresolvedResolutionnot fixable 
PlatformPCOSWindowsOS Version10
Product Version 
Target VersionFixed in Version 
First NameIrene
Last NameMutuku
Emailmutukumirene@gmail.com
Confirm Emailmutukumirene@gmail.com
Summary0001239: ERROR MESSAGE: Error-Allignement Failed: MUSCLE Log file did not end properly, suggesting an unhandled exception
DescriptionReport bugs or other issues regarding the MEGA software
I'm using the MEGA X sofware downloaded, Windows 10
I am trying to align reference sequences and my sequences and also get phylogeny tree.
I get an error message
The error message is
Error-Allignement Failed: MUSCLE Log file did not end properly, suggesting an unhandled exception.

I have a attached the fasta file with the reads. I hope you can explain what is wrong as I have another file giving the same error.
Steps To ReproduceSelected Align | Edit/Build Alignment from the launch bar.
Select Create a new alignment and then select DNA.
From the Alignment Explorer window, I selected Data | Open | Retrieve sequences from a
file and select the “k1-8.txt” file (attached).
On the Alignment Explorer main menu, I selected Edit | Select All.
On the Alignment Explorer i selected the icon that looks like a flexing arm.
And select Align DNA.


Additional Informationurgently help as I haveanother folder to work on too
TagsNo tags attached.
Relationships
Attached Filestxt k1-k8_refgenomesfasta.txt (965,653) 2019-05-21 03:10
https://megasoftware.net/mantis_bt/
Issue History
Date ModifiedUsernameFieldChange
2019-05-21 03:10guestNew Issue
2019-05-21 03:10guestFile Added: k1-k8_refgenomesfasta.txt
2019-05-21 08:58gstecherNote Added: 0004249
2019-05-21 08:58gstecherStatusnew => resolved
2019-05-21 08:58gstecherResolutionopen => not fixable
2019-05-21 08:58gstecherAssigned To => gstecher

Notes
(0004249)
gstecher   
2019-05-21 08:58   
Hi Irene,

I am writing in response to the bug report you submitted regarding the MEGA software. The alignment fails with your data file because the MUSCLE program is not able to allocate enough memory (MUSCLE on Windows is a 32-bit application) to complete the analysis. To get around this, you can download and use 64-bit MUSCLE for macOS or Linux. Or you could use something like MAFFT which is designed to align larger data sets.

--
Best regards,
Glen Stecher
Institute for Genomics and Evolutionary Medicine
igem.temple.edu