MantisBT - MEGA
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IDProjectCategoryView StatusDate SubmittedLast Update
0001258MEGATimetree Wizardpublic2019-06-04 06:332023-04-14 18:34
Reporterguest 
Assigned Togstecher 
PrioritynormalSeverityminorReproducibilityhave not tried
StatusassignedResolutionopen 
PlatformOSOS Version
Product VersionMEGA 11 (Graphical Interface version) 
Target VersionFixed in Version 
First NameFelipe
Last NameFreitas
Emailfelipevieirafreitas@gmail.com
Confirm Emailfelipevieirafreitas@gmail.com
Summary0001258: Problems inserting calibration points in RelTime
DescriptionI have been trying to calculate divergence times using Mega in a UCEs database with 0000987:0000900 loci and ~400000bp, but the software is not allowing me to include calibration points. I have been trying in a Windows and Ubuntu platform, but I am always receiving the following message: "The following divergence time constraints were disregarded because they are invalid". This message is appearing independent of the kind of calibration point inserted.
Steps To ReproduceI am opening the matrix in .meg format (it was converted in Mega from a nexus file), and them selecting the RelTime option in the Clocks menu, and then following the tutorial in time tree wizard, selecting a tree, defining an outgroup, and finally adding calibration points, where the problems appear.
Additional InformationI have run analysis without calibration points using Mega CC and it worked fine, but, adding calibration points would be the best option for what I am intending to do.
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Issue History
Date ModifiedUsernameFieldChange
2019-06-04 06:33guestNew Issue
2019-06-04 12:34gstecherAssigned To => gstecher
2019-06-04 12:34gstecherStatusnew => assigned
2019-06-04 12:34gstecherNote Added: 0004256
2022-12-05 06:58guestNote Added: 0005897
2023-04-14 18:34guestNote Added: 0006445

Notes
(0004256)
gstecher   
2019-06-04 12:34   
Hi Felipe,

I am writing in response to your question regarding the MEGA software. In order to understand the problem I need more information. Can you send me the all the input data (meg file, tree file, calibrations used) you are using so I can duplicate the issue?

--
Best regards,
Glen Stecher
Institute for Genomics and Evolutionary Medicine
igem.temple.edu
(0005897)
guest   
2022-12-05 06:58   
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(0006445)
guest   
2023-04-14 18:34   
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