MantisBT - MEGA
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0001259MEGA[All Projects] Feedbackpublic2019-06-04 21:022019-06-07 13:09
guest 
gstecher 
normalminorhave not tried
resolvednot fixable 
 
 
heng
Ku
kuheng3@gmail.com
kuheng3@gmail.com
0001259: Error Alignment Failed
Hi
I tried to run 134 whole genome DNA sequence (100kbp) alignment using MUSCLE (neighbor-joining). I keep getting this message "Error Alignment failed: Muscle logfile didn't end properly, suggesting an unhandled exception." However, If i tried 12 sequence on the same protein, the program will work fine. Is there any way I can solve this problem. Thank you for your time to read this email.

Kind Regards,
Heng Ku
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Issue History
2019-06-04 21:02guestNew Issue
2019-06-07 13:09gstecherNote Added: 0004257
2019-06-07 13:09gstecherStatusnew => resolved
2019-06-07 13:09gstecherResolutionopen => not fixable
2019-06-07 13:09gstecherAssigned To => gstecher

Notes
(0004257)
gstecher   
2019-06-07 13:09   
Hi Heng,

I am writing in response to the bug report you recently submitted regarding the MEGA software. Unfortunately, MEGA is limited in the amount of data it can perform sequence alignment on. You want try MAFFT, which is more suitable for such a large data set.

--
Best regards,
Glen Stecher
Institute for Genomics and Evolutionary Medicine
igem.temple.edu