MantisBT - MEGA
View Issue Details
0001510MEGA[All Projects] Feedbackpublic2019-10-24 08:572019-10-25 10:48
guest 
gstecher 
normalminorhave not tried
resolvedno change required 
MEGA 11 (Graphical Interface version) 
 
Connie
Roderick
croderick@usgs.gov
croderick@usgs.gov
0001510: Reverse Complement
When adding a fasta file into mega, it sometimes switches the sequences to plus/plus and to know this for future alignments w/ other sequences, I need to BLAST it so I know if I need to change it to plus/minus to match other seqs in the alignment.

Is there a way to add seq's from fasta files w/o having to blast each one each time added to find out if it needs to have "reverse complement" applied so that all sequences in the alignment are the same plus/minus?

 
No tags attached.
Issue History
2019-10-24 08:57guestNew Issue
2019-10-25 10:48gstecherNote Added: 0004321
2019-10-25 10:48gstecherStatusnew => resolved
2019-10-25 10:48gstecherResolutionopen => no change required
2019-10-25 10:48gstecherAssigned To => gstecher

Notes
(0004321)
gstecher   
2019-10-25 10:48   
Hi Connie,

I am writing in response to your question regarding the MEGA software. MEGA does not automatically reverse complement any sequence data. You can test this by opening your fasta file in a text editor and comparing it to the display in MEGA.

--
Best regards,
Glen Stecher
Institute for Genomics and Evolutionary Medicine
igem.temple.edu