MantisBT - MEGA
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0001585MEGA[All Projects] Feedbackpublic2019-11-21 10:312019-11-21 11:47
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gstecher 
normalminorhave not tried
resolvedno change required 
MEGA 11 (Graphical Interface version) 
 
Francois
Robin
francois.robin@upmc.fr
francois.robin@upmc.fr
0001585: Multiple sequence input
Hi,
I'm trying to use MEGA to make a tree and an alignment, starting from a curated multi-fasta file containing a large number of sequences (>1000).
Each sequence in the unique text file is preceded by a header, for example >Acas_1_10
How can I input a this large number of sequences ? Would it be possible to create a single MEGA file containing all sequences ? Or do I have to create an individual file for each protein to be aligned?
Thanks in advance for your help!
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Issue History
1969-12-31 17:33ddviewNew Issue
2019-11-21 10:31guestNew Issue
2019-11-21 11:47gstecherNote Added: 0004336
2019-11-21 11:47gstecherStatusnew => resolved
2019-11-21 11:47gstecherResolutionopen => no change required
2019-11-21 11:47gstecherAssigned To => gstecher

Notes
(0004336)
gstecher   
2019-11-21 11:47   
Hi Francois,

I am writing in response to your question regarding the MEGA software. Are you trying to concatenate multiple gene sequences to create a single alignment file? If so, there is an option in MEGAX to concatenate multiple fasta files. First, you copy all the files you want to concatenate into a single directory. Then on the MEGAX main form, click Data->Concatenate Sequence Alignments. MEGAX will prompt you for the directory containing your fasta files and then try and concatenate them.

--
Best regards,
Glen Stecher
Institute for Genomics and Evolutionary Medicine
igem.temple.edu