MantisBT - MEGA
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IDProjectCategoryView StatusDate SubmittedLast Update
0002406MEGA[All Projects] Feedbackpublic2020-12-04 12:282021-01-05 15:21
Reporterguest 
Assigned Togstecher 
PrioritynormalSeverityminorReproducibilityhave not tried
StatusresolvedResolutionnot fixable 
PlatformOSOS Version
Product VersionMEGA 11 (Graphical Interface version) 
Target VersionFixed in Version 
First NameAurelien
Last NameLorin
Emaillorina@medicago.com
Confirm Emaillorina@medicago.com
Summary0002406: Not all RAM used
DescriptionHello,
using Mega7 for sequences aligment on a Virtual Machine, Mega7 does not use all the RAM available (only 4 Mo despite I indicated 64Mo) and stop with an error message that there is not enough memory for the calculus.
Using MegaX, I have an error message "Error-Aligmment: MUSCLE Log File did not end properly, suggesting an unhandled exception" which seems related also to not enough RAM according to forums.
Please could you help me to solve this problem?
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Date ModifiedUsernameFieldChange
2020-12-04 12:28guestNew Issue
2021-01-05 15:21gstecherNote Added: 0004540
2021-01-05 15:21gstecherStatusnew => resolved
2021-01-05 15:21gstecherResolutionopen => not fixable
2021-01-05 15:21gstecherAssigned To => gstecher

Notes
(0004540)
gstecher   
2021-01-05 15:21   
Hi Aurelien,
I am writing in response to your question regarding MUSCLE alignment using the MEGA software. Unfortunately, the developer of MUSCLE only distributes a 32-bit version for Windows so it is not able to utilize all the memory available on your system. To get around there are a couple of options:

    If you have access to a 64-bit Linux or macOS computer you can download a 64-bit version of MUSCLE for that system and run MUSCLE there
    You can use something like MAFFT which is designed for aligning large sequence data


Best regards,
Glen Stecher
Institute for Genomics and Evolutionary Medicine
igem.temple.edu