MantisBT - MEGA
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0002481MEGAAlignment Explorerpublic2021-01-12 20:012021-01-14 10:12
guest 
gstecher 
normalminorhave not tried
resolvedfixed 
PCWindows10
MEGA 11 (Graphical Interface version) 
 
Zaki
Suficiencia
zaki_suficiencia@dlsu.edu.ph
zaki_suficiencia@dlsu.edu.ph
0002481: MUSCLE Alignment Output is Different Between Operating Systems
The same two sequences were aligned using the MUSCLE algorithm, by codon, with gaps not deleted. The alignment outputs were different between MEGA for Windows 10 and MEGAX for OS X Catalina.

The same two sequences were aligned using the MUSCLE algorithm, by codon, with gaps not deleted. The alignment was different between MEGA for Windows 10 and MEGAX for OS X Catalina.
1. Open two .ab1 files in MEGA
2. For both files, press "add unmasked sequence to alignment explorer"
3. Get the reverse complement of F2_F2R
4. Align using MUSCLE by codon, with gaps not deleted.
The zip file attached contains 4 files:
Two .ab1 files for a contig assembly activity
Two .mas alignment session files with the output of the MUSCLE algorithm for Windows and OS X
No tags attached.
zip MUSCLE bug.zip (283,935) 2021-01-12 20:01
https://megasoftware.net/mantis_bt/
Issue History
2021-01-12 20:01guestNew Issue
2021-01-12 20:01guestFile Added: MUSCLE bug.zip
2021-01-14 10:12gstecherNote Added: 0004552
2021-01-14 10:12gstecherStatusnew => resolved
2021-01-14 10:12gstecherResolutionopen => fixed
2021-01-14 10:12gstecherAssigned To => gstecher

Notes
(0004552)
gstecher   
2021-01-14 10:12   
fixed in 10.2.3