MantisBT - MEGA
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0002748MEGAAlignment Explorerpublic2021-04-17 11:242021-04-20 11:06
guest 
gstecher 
normalminorhave not tried
resolvedno change required 
PCWindows10
MEGA 11 (Graphical Interface version) 
 
Janos
Agoston
agoston.janos123@gmail.com
agoston.janos123@gmail.com
0002748: invalid base found: ? (in line 172)
In version 10.2.4 I wanted to do a Phylogenetic Analysis from the attached file to find the best fit DNA model. When cliclikg on the Phlylogenetic Analysis menu I get this error message: invalid base found: ? (in line 172).
Can you help me please what causes this problem?
I have chosen Invertebrate mitochondrion as genetic code. But the same happens with standard code too.
No tags attached.
? Cacopsylla_COI nt CW.mas (47,589) 2021-04-17 11:24
https://megasoftware.net/mantis_bt/
Issue History
1969-12-31 17:33Nikita VikhrevNote Added: 0002166
1969-12-31 17:33user19Note Added: 0002174
1969-12-31 17:33user19Statusnew => assigned
1969-12-31 17:33user19Assigned To => user19
2021-04-17 11:24guestNew Issue
2021-04-17 11:24guestFile Added: Cacopsylla_COI nt CW.mas
2021-04-20 11:06gstecherNote Added: 0004576
2021-04-20 11:06gstecherStatusnew => resolved
2021-04-20 11:06gstecherResolutionopen => no change required
2021-04-20 11:06gstecherAssigned To => gstecher

Notes
(0002166)
Nikita Vikhrev   
1969-12-31 17:33   
Hi Dan,

Please ask the user to send the newick file causing the problem. Thanks.

Koichiro
(0002174)
user19   
1969-12-31 17:33   
Sent user email on 3/30 (the day bug was submitted) I have not yet heard back from them.

Sent the user a second email asking for a response.
(0004576)
gstecher   
2021-04-20 11:06   
Hi Janos,

I am writing in response to your question regarding the MEGA software. Somehow, in the alignment file you uploaded, two sequences have an odd character at the last position (they are colored grey in the Alignment Explorer - see screen shot below). If you delete those two bases, the analysis will work.