MantisBT - MEGA
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0002846MEGA[All Projects] Feedbackpublic2021-05-25 17:382021-05-27 07:27
guest 
gstecher 
normalminorhave not tried
resolvedwon't fix 
MEGA 11 (Graphical Interface version) 
 
Dipon
Saha
dipon1992saha@student.ubc.ca
dipon1992saha@student.ubc.ca
0002846: muscle error
Hi
I am Dipon, student of university of british coloumbia. I am trying to use this software for alignment of DNA sequence. I have 2000 DNA sequence. Whenever I am trying to align these sequences using muscle the software showing me this error. " Error-Alignment failed: Muscle log file did not end properly, suggesting an unhandled exception." please let me know how to over come these problem.

Thanking you in advance and looking forward to hear from you
kind regards,
Dipon
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Issue History
1969-12-31 17:33user614Note Added: 0002302
1969-12-31 17:33user614Statusnew => acknowledged
1969-12-31 17:33user614Statusacknowledged => closed
2021-05-25 17:38guestNew Issue
2021-05-27 07:22gstecherNote Deleted: 0002302
2021-05-27 07:22gstecherFile Deleted: crash-report.txt
2021-05-27 07:27gstecherNote Added: 0004597
2021-05-27 07:27gstecherStatusnew => resolved
2021-05-27 07:27gstecherResolutionopen => won't fix
2021-05-27 07:27gstecherAssigned To => gstecher

Notes
(0004597)
gstecher   
2021-05-27 07:27   
Hi Dipon,

I am writing in response to the bug report you recently submitted regarding the MEGA software. MUSCLE is actually a separate software that MEGA uses for sequence alignment. Because it is a separate software, we are not able to debug it and see why the alignment failed. Also, MUSCLE is not designed for use with very large alignments. You should probably use something like MAFFT for sequence alignment as it is engineered for large data.

Best regards,
Glen Stecher
Institute for Genomics and Evolutionary Medicine
igem.temple.edu