MantisBT - MEGA
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0000031MEGA[All Projects] Suggestionpublic2016-02-05 06:442016-02-05 15:01
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gstecher 
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MEGA-CC 11 (command line version) 
 
Themis
Giannoulis
themisgia@hotmail.com
themisgia@hotmail.com
0000031: Genetic codes
Hi

I am using the command line version on some mitochondrial sequences and I want to test for selection using Z-test but I have a problem because I can't change the genetic code on megaproto, and I am getting stop codons in my sequences. Is there a way to manipulate the file or the .mao file so it can change to mitochondrial genetic code?
Thanks in advance
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Issue History
2016-02-05 06:44guestNew Issue
2016-02-05 15:01gstecherAssigned To => gstecher
2016-02-05 15:01gstecherStatusnew => assigned
2016-02-05 15:01gstecherNote Added: 0003590

Notes
(0003590)
gstecher   
2016-02-05 15:01   
Hi Themis,

I am writing in response to the question you recently posted to the MEGA bug tracker regarding how to set the genetic code table for the z-test of selection analysis. Thanks for the feedback. We will fix the bug you found soon. In the meantime, I think you can work around the problem as follows:

    Add a couple of lines for the genetic code table to the .mao file as in the attached example.
    If you need a different code table from the one in the example then:
        open megaproto
        select coding data
        select some other analysis (like Phylogeny->Construct/Test Maximum Likelihood Tree...)
        set the Substitution Type to Amino Acid
        select the desired genetic code table
        save that mao file
        open that mao file in a text editor and copy the two code table lines from that file
        paste the two code table lines into your .mao file for the z-test

--
Best regards,

Glen Stecher