MantisBT - MEGA
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IDProjectCategoryView StatusDate SubmittedLast Update
0000323MEGAAlignment Explorerpublic2016-12-01 00:062016-12-02 07:28
Reporterguest 
Assigned Togstecher 
PrioritynormalSeverityminorReproducibilityhave not tried
StatusresolvedResolutionno change required 
PlatformPCOSWindowsOS VersionVista
Product VersionMEGA 11 (Graphical Interface version) 
Target VersionFixed in Version 
First NameSubho
Last NameGhosh
Emailsubho@mbu.iisc.ernet.in
Confirm Emailsubho@mbu.iisc.ernet.in
Summary0000323: error is coming while trying to make phylogenetic tree. tree
DescriptionAn error" all sequences must be of equal length" is coming whike all the sequences are indeed of equal length and here the error is showing at the name of the sequence line. I changed the names but still the error is not going.
Steps To Reproduce
Additional Information
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Issue History
Date ModifiedUsernameFieldChange
2016-12-01 00:06guestNew Issue
2016-12-01 13:11gstecherAssigned To => gstecher
2016-12-01 13:11gstecherStatusnew => assigned
2016-12-01 13:11gstecherNote Added: 0003721
2016-12-02 07:28gstecherNote Added: 0003722
2016-12-02 07:28gstecherStatusassigned => resolved
2016-12-02 07:28gstecherResolutionopen => no change required

Notes
(0003721)
gstecher   
2016-12-01 13:11   
Hi Subho,

I am writing in response to the bug report you recently submitted regarding the MEGA software. Could you send me the alignment file you were using when the error occurred so that I can reproduce the error?

--
Best regards,

Glen Stecher
Institute for Genomics and Evolutionary Medicine
igem.temple.edu
(0003722)
gstecher   
2016-12-02 07:28   
Hi Subho,

Thanks for sending that file. But the sequences are not of equal length and so it is not possible to make valid comparisons between them. For example, consider the following two sequences in the file:

#sp1|P10275|ANDR_HUMAN
PPQKT---CLICGDEASGCHY---------------------------GALTCGSCKVFF
------

#tr1|A4GT83|A4GT83_DANRE
-PQRT---CLICSDEASGCHY---------------------------GALTCGSCKVFF
-QKYL---CASRNDCTIDKL----------------------------RRKNCPSCRLKK
------
------

On 12/1/2016 10:46 PM, subho@mbu.iisc.ernet.in wrote:
> Dear Glen,
> The file is attached with the mail.
>
> Regards,
> Subho
>