MantisBT - MEGA
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IDProjectCategoryView StatusDate SubmittedLast Update
0003685MEGADistance Data Explorerpublic2022-05-16 07:392023-04-14 18:52
Reporterguest 
Assigned To 
PrioritynormalSeverityminorReproducibilityhave not tried
StatusnewResolutionopen 
PlatformPCOSWindowsOS Version10
Product VersionMEGA 11 (Graphical Interface version) 
Target VersionFixed in Version 
First NameBalázs
Last NameVajna
Emailvajna.balazs@ttk.elte.hu
Confirm Emailvajna.balazs@ttk.elte.hu
Summary0003685: Problem in distance calcuation
DescriptionDear Mega11 programmers,
I have an aligned set of sequences (see attached), for which I wanted to construct a NJ-tree in Mega11 (Build#: 11220201-x86_64) using Tamura 3-parameter model, but it failed.

I'd be curious, what could be the problem

Thanks for your help:

Balázs Vajna, PhD, assistant professor
Department of Microbiology
Eötvös Loránd University, Budapest, Hungary
Homepage: mikrobi.elte.hu/vajna_balazs_en
Steps To Reproduce1, I've loaded the attached fasta file to Mega11 with "Analyze" option as nucleotid, non-protein coding sequence.
2, Then I started the Phylogeny / Construct/Test Neighbor-Joining Tree
3, I've set the following paramaters:
- Substitution model: Tamura 3-parameter model including transitions and transversions
- using uniform rates with homogenous patterns
- missing data: pairwise deletions
4, After starting the calculation I've received the following error message:

Distance calculation failed - distance matrix could not be computed. Error id is 4518 (Tamura-distance incalculable for some pairs.)
Additional Information
TagsNo tags attached.
Relationships
Attached Files? Rizobium_atpD_probe_ARB_aligned.fas (7,931) 2022-05-16 07:39
https://megasoftware.net/mantis_bt/
Issue History
Date ModifiedUsernameFieldChange
2022-05-16 07:39guestNew Issue
2022-05-16 07:39guestFile Added: Rizobium_atpD_probe_ARB_aligned.fas
2023-04-14 18:52guestNote Added: 0006669

Notes
(0006669)
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