MantisBT - MEGA
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0004089MEGAAlignment Explorerpublic2022-11-16 10:462023-04-14 18:44
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normalminorhave not tried
newopen 
PCWindows10
 
 
Matthew
Cserhati
csmatyi@protonmail.com
csmatyi@protonmail.com
0004089: don't delete underscores in fasta header during alignment!
Hello,

When MEGA aligns nucleotide sequences, it erases the spaces in the header row. So, if there is a species name such as >Felis catus, in the alignment only Felis will show up.

This is a problem if I am studying multiple Felis species, i.e. Felis catus, Felis domesticus, Felis chaus, Felis margarita (the sand cat).

When I want to read the alignment file into R, it produces an error message, because these 4 species will have the same name, Felis.

Thanks, Matt
Align the attached fasta file (sequences.fasta), using ClustalW in the MEGA software. Double check to see that only Felis or the first name ends up in the alignment file.
No tags attached.
? sequences.fasta (102,945) 2022-11-16 10:46
https://megasoftware.net/mantis_bt/
Issue History
1969-12-31 17:33user614Note Added: 0002806
1969-12-31 17:33user614Statusnew => closed
1969-12-31 17:33user614Resolutionopen => won't fix
2022-11-16 10:46guestNew Issue
2022-11-16 10:46guestFile Added: sequences.fasta
2023-04-14 18:44guestNote Added: 0006574

Notes
(0002806)
user614   
1969-12-31 17:33   
Hello Monika,

I am writing in response to the error report you recently submitted to the MEGA bug tracker. Unfortunately, MEGA is a native Windows application and does not perform optimally for all Mac users. For those Mac users which experience instability issues when using MEGA, we recommend that they download the Windows version of MEGA and run it using emulation or virtualization software such as VMWareFusion.


Best regards,

Glen Stecher
(0006574)
guest   
2023-04-14 18:44   
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