MantisBT - MEGA
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IDProjectCategoryView StatusDate SubmittedLast Update
0000442MEGA[All Projects] Feedbackpublic2017-04-26 08:322017-05-12 12:41
Reporterguest 
Assigned Togstecher 
PrioritynormalSeverityminorReproducibilityhave not tried
StatusresolvedResolutionno change required 
PlatformOSOS Version
Product Version 
Target VersionFixed in Version 
First NameHaneen
Last NameSarairah
Emailhanin.sarairah@gmail.com
Confirm Emailhanin.sarairah@gmail.com
Summary0000442: Sequences alignment
DescriptionDear Sir,

I used MEGA version 7 for nucleotide alignment and phylogenetic analysis. I tried to align sequences from genbank with my samples. I observe the alignment changed when i add more samples with huge differences for example sample (Y) located in group 1 (from genbank), but when i add more samples and aligned them the sequence (Y) now become with group 3 (from genbank).

Is this make sense or i missed something?

I look forward to hearing from you

Best regards,
Haneen
Master's Student
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Date ModifiedUsernameFieldChange
2017-04-26 08:32guestNew Issue
2017-05-12 12:41gstecherNote Added: 0003799
2017-05-12 12:41gstecherStatusnew => resolved
2017-05-12 12:41gstecherResolutionopen => no change required
2017-05-12 12:41gstecherAssigned To => gstecher

Notes
(0000489)
paul   
1969-12-31 17:33   
Suggestion is saved and closed.
(0003799)
gstecher   
2017-05-12 12:41   
Hi Haneen,

I am writing in response to your question regarding the MEGA software. You should use the tools available in MEGA to asses the robustness of the results. For instance, you should use the bootstrap test for phyogenetic tree construction. Additionally, it may be advisable to use the model selection test to select and appropriate model, then use ML tree construction which is more robust than the distance methods.

--
Best regards,

Glen Stecher
Institute for Genomics and Evolutionary Medicine
igem.temple.edu