MantisBT - MEGA
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IDProjectCategoryView StatusDate SubmittedLast Update
0004554MEGATree Explorerpublic2023-02-15 19:252023-04-14 00:44
Reporterguest 
Assigned To 
PrioritynormalSeverityminorReproducibilityhave not tried
StatusnewResolutionopen 
PlatformPCOSWindowsOS Versionother
Product VersionMEGA 11 (Graphical Interface version) 
Target VersionFixed in Version 
First NameNIK SHAWN
Last NameTABAO
Emailnctabao@up.edu.ph
Confirm Emailnctabao@up.edu.ph
Summary0004554: Crashing while phylotree reconstruction
DescriptionThe MEGA 11 software crashes or shuts down in Microsoft Windows 11 during bootstrapping when generating a maximum likelihood tree with specific settings indicated in the "steps to reproduce" field. Replicated the same settings in MEGA X but the older app did not crash. I tried using different computers with different computing power but the same experience.
Steps To Reproduce1. Alignment of the dataset with 100 sequences of 16S rRNA genes via MUSCLE (see Combined.mas file attached)
2. Enter Phylogenetic Analysis mode.
3. Construct a Maximum Likelihood tree via settings:
    a. ML statistical method
    b. Bootstrap method.
    c. 1000 replications
    d. Nucleotide Substitution
    e. Tamura-Nei Model
    f. Gamma Distributed with Invariant Sites (G+I)
    g. 5 discrete gamma categories
    h. all sites are used for data treatment
    i. default codon positions (all checked)
    j. NNI ML heuristic method
    k. default initial tree
    l. branch swap filter none
    m. 4-8 Number of threads
Additional InformationI have attached the MUSCLE-generated alignment (combined.mas) for your reference.
TagsNo tags attached.
Relationships
Attached Files? Combined.mas (174,872) 2023-02-15 19:25
https://megasoftware.net/mantis_bt/
Issue History
Date ModifiedUsernameFieldChange
2023-02-15 19:25guestNew Issue
2023-02-15 19:25guestFile Added: Combined.mas
2023-04-14 00:44guestNote Added: 0006245

Notes
(0006245)
guest   
2023-04-14 00:44   
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