MantisBT - MEGA
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0005170MEGATree Explorerpublic2023-06-07 05:042023-06-07 05:04
guest 
 
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PCWindows10
MEGA 11 (Graphical Interface version) 
 
Alejandro
Giraldo Fonseca
alejandro.giraldo@botinst.uzh.ch
alejandro.giraldo@botinst.uzh.ch
0005170: MEGA11 crashes in Windows11
As soon as I try to do Neighbor-Joining Tree (bootstrap = ON x1000) after MUSCLE alignment, the program crashes and disappear, leaving no warning or error report.
Open the software:
Align > Build new alignment > Protein > remove sequence 0 > add sequences (attached below) > Make MUSCLE aligment > export aligment as MEGA > close aligment windows > Phylogeny > COnstruct /test neighbor joining tree > activate bootstrap > set to 500 or whatever > rest by default > Press Ok > CRASH
Peptides to align:
>MpFER
MKITEGRFRLSLLLLLLLISAATLISAADYSPTEKILLNCGGGASNLTDTDNRIWISDVKSKFLSSSSEDSKTSPALTQDPSVPEVPYMTARVFRSPFTYTFPVASGRKFVRLYFYPNSYDGLNATNSLFSVSFGPYTLLKNFSASQTAEALTYAFIIKEFVVNVEGGTLNMTFTPESAPSNAYAFVNGIEVTSMPDMYSSTDGTLTMVGSSGSVTIDNSTALENVYRLNVGGNDISPSADTGLYRSWYDDQPYIFGAGLGIPETADPNMTIKYPTGTPTYVAPVDVYSTARSMGPTAQINLNYNLTWIFSIDSGFTYLVRLHFCEVSSNITKINQRVFTIYLNNQTAEPEADVIAWTSSNGVPFHKDYVVNPPEGNGQQDLWLALHPNPVNKPEYYDSLLNGVEIFKMNTSDGNLAGTNPIPGPQVTADPSKVLRPTTRKSKSNTAIIAGAASGAVVLALIIGFCVFGAYRRRKRGDYQPASDATSGWLPLSLYGNSHSAGSAKTNTTGSYASSLPSNLCRHFSFAEIKAATKNFDESRVLGVGGFGKVYRGEIDGGTTKVAIKRGNPMSEQGVHEFQTEIEMLSKLRHRHLVSLIGYCEENCEMILVYDYMAHGTMREHLYKTQNPSLPWKQRLEICIGAARGLHYLHTGAKHTIIHRDVKTTNILLDEKWVAKVSDFGLSKTGPTLDHTHVSTVVKGSFGYLDPEYFRRQQLTEKSDVYSFGVVLFEALCARPALNPTLAKEQVSLAEWAPYCYKKGMLDQIVDPYLKGKITPECFKKFAETAMKCVLDQGIERPSMGDVLWNLEFALQLQESAEENGKGVCGDMDMDEIKYDDGNCKGKNDKSSDVYEGNVTDSRSSGIDMSIGGRSLASEDSDGLTPSAVFSQIMNPKGR

>AtANXUR1
MSGKTRILFFLTCLSFLLVFPTRSNGQDLALSCGTSEASADQDKKKWEPDTKFLKTGNSIHATATYQDPSLLSTVPYMTARIFTAPATYEIPIKGDKRHLLRLYFYPSTYTGLNISNSYFTVEANDVTLLSNFSAAITCQALTQAYLVKEYSLAPTDKDVLSIKFTPSDKYRDAFAFINGIEVIQMPELFDTAALVGFTDQTMDAKTANLQSMFRLNVGGQDIPGSQDSGGLTRTWYNDAPYIFSAGLGVTLQASNNFRINYQNMPVSIAPADIYKTARSQGPNGDINLKSNLTWMFQIDKNFTYILRLHFCEFQLSKINQKVFNIYINNRTAQADTTPADIIGWTGEKGIPMYKDYAIYVDANNGGEEITLQMTPSTFGQPEYYDSSLNGLEIFKMDTMKNLAGPNPEPSPMQAEEEVKKEFKNEKRHAFIIGSAGGVLAVLIGALCFTAYKKKQGYQGGDSHTSSWLPIYGNSTTSGTKSTISGKSNNGSHLSNLAAGLCRRFSLPEIKHGTQNFDDSNVIGVGGFGKVYKGVIDGTTKVAVKKSNPNSEQGLNEFETEIELLSRLRHKHLVSLIGYCDEGGEMCLVYDYMAFGTLREHLYNTKKPQLTWKRRLEIAIGAARGLHYLHTGAKYTIIHRDVKTTNILVDENWVAKVSDFGLSKTGPNMNGGHVTTVVKGSFGYLDPEYFRRQQLTEKSDVYSFGVVLFEILCARPALNPSLPKEQVSLGDWAMNCKRKGNLEDIIDPNLKGKINAECLKKFADTAEKCLNDSGLERPTMGDVLWNLEFALQLQETADGTRHRTPNNGGSSEDLGRGGMAVNVAGRDDVSDLSSEDNTEIFSQIVNPKGR

>AtTHE1
MVFTKSLLVLLWFLSCYTTTTSSALFNPPDNYLISCGSSQNITFQNRIFVPDSLHSSLVLKIGNSSVATSTTSNNSTNSIYQTARVFSSLASYRFKITSLGRHWIRLHFSPINNSTWNLTSASITVVTEDFVLLNNFSFNNFNGSYIFKEYTVNVTSEFLTLSFIPSNNSVVFVNAIEVVSVPDNLIPDQALALNPSTPFSGLSLLAFETVYRLNMGGPLLTSQNDTLGRQWDNDAEYLHVNSSVLVVTANPSSIKYSPSVTQETAPNMVYATADTMGDANVASPSFNVTWVLPVDPDFRYFVRVHFCDIVSQALNTLVFNLYVNDDLALGSLDLSTLTNGLKVPYFKDFISNGSVESSGVLTVSVGPDSQADITNATMNGLEVLKISNEAKSLSGVSSVKSLLPGGSGSKSKKKAVIIGSLVGAVTLILLIAVCCYCCLVASRKQRSTSPQEGGNGHPWLPLPLYGLSQTLTKSTASHKSATASCISLASTHLGRCFMFQEIMDATNKFDESSLLGVGGFGRVYKGTLEDGTKVAVKRGNPRSEQGMAEFRTEIEMLSKLRHRHLVSLIGYCDERSEMILVYEYMANGPLRSHLYGADLPPLSWKQRLEICIGAARGLHYLHTGASQSIIHRDVKTTNILLDENLVAKVADFGLSKTGPSLDQTHVSTAVKGSFGYLDPEYFRRQQLTEKSDVYSFGVVLMEVLCCRPALNPVLPREQVNIAEWAMAWQKKGLLDQIMDSNLTGKVNPASLKKFGETAEKCLAEYGVDRPSMGDVLWNLEYALQLEETSSALMEPDDNSTNHIPGIPMAPMEPFDNSMSIIDRGGVNSGTGTDDDAEDATTSAVFSQLVHPRGR

>AtFER
MKITEGRFRLSLLLLLLLISAATLISAADYSPTEKILLNCGGGASNLTDTDNRIWISDVKSKFLSSSSEDSKTSPALTQDPSVPEVPYMTARVFRSPFTYTFPVASGRKFVRLYFYPNSYDGLNATNSLFSVSFGPYTLLKNFSASQTAEALTYAFIIKEFVVNVEGGTLNMTFTPESAPSNAYAFVNGIEVTSMPDMYSSTDGTLTMVGSSGSVTIDNSTALENVYRLNVGGNDISPSADTGLYRSWYDDQPYIFGAGLGIPETADPNMTIKYPTGTPTYVAPVDVYSTARSMGPTAQINLNYNLTWIFSIDSGFTYLVRLHFCEVSSNITKINQRVFTIYLNNQTAEPEADVIAWTSSNGVPFHKDYVVNPPEGNGQQDLWLALHPNPVNKPEYYDSLLNGVEIFKMNTSDGNLAGTNPIPGPQVTADPSKVLRPTTRKSKSNTAIIAGAASGAVVLALIIGFCVFGAYRRRKRGDYQPASDATSGWLPLSLYGNSHSAGSAKTNTTGSYASSLPSNLCRHFSFAEIKAATKNFDESRVLGVGGFGKVYRGEIDGGTTKVAIKRGNPMSEQGVHEFQTEIEMLSKLRHRHLVSLIGYCEENCEMILVYDYMAHGTMREHLYKTQNPSLPWKQRLEICIGAARGLHYLHTGAKHTIIHRDVKTTNILLDEKWVAKVSDFGLSKTGPTLDHTHVSTVVKGSFGYLDPEYFRRQQLTEKSDVYSFGVVLFEALCARPALNPTLAKEQVSLAEWAPYCYKKGMLDQIVDPYLKGKITPECFKKFAETAMKCVLDQGIERPSMGDVLWNLEFALQLQESAEENGKGVCGDMDMDEIKYDDGNCKGKNDKSSDVYEGNVTDSRSSGIDMSIGGRSLASEDSDGLTPSAVFSQIMNPKGR

>PaRALF1
LLRRVRYYISYGALSANRVPCRPRSGRSYYTRNCYRATGPVRPYHRSCTAITRCRRYTS

>PaRALF2
LLRRVRYYISYGALSANRVPCRPRSGRSYYTRNCYRATGPVRPYRRSCTAITRCRRYTT

>PaRALF3
LLRRIHYYISYGALAANHVPCRPRSGRSYYTRNCYSATGPVRPYHRSCTAITRCRRYTS

>PaRALF4
RLLRQIRYYISYGALIANRIPCPPRSGRSYYTRNYYRATEPVKPYHRSCTAITRCLRDTI

>PaRALF5
LLRRVRYYISYSALYADSVNCPPRSGRSYYTRDCFRATGPVRPYRRSCTTITRCRRYTD

>PaRALF6
LLRRVRYYISYGALAANRVPCRPRSGRMQLVAPLVLLLVSLFCMADSFNDQELTVTSDWLGGVASYEFDSFAGQICDGTLGDCNDDNGEFMMDTEAPGR

>PaRALF6.2
LLRRVRYDISYGALAANRVPCRPRSGRSYYTRNCYRATGPVKPYRRSCTAIT

>PaRALF7
LLRRVRYYISYGALAANRVPCRPRSGRSYYTRNCYAATGPRLVREIAHDFKTDLRFQSSTVAALQKVAEAYLVGLFKDTNMCAIHAKRVTIMPKDIQLTRHIRGERA

>PaRALF8
LWWRRTYISYDALSRDRVPCSKRGTSYYNCGRTSQANPYNRGCTQITRCARDTG

>PaRALF9
SIIICHGDECGLYDENDNEGVMVYINTRRKMTESRTVQHISYGALAADNVPCPITGQSYYYCNTSDPNVNPYTRACTAITRCERDTN

>PaRALF10
QVSYGLQCDPNAEECDLVQAEATVDSVYTFPEHSEEGEGEVSSRRRTMSNETQPVPYISYAALSANNVPCPISGQSYYHCNYSDSAVNPYTRSCTAIARCERDVN

>PpRALF1.1
QAPFAAVAPSPFTGFPPQEAPAFAGSGGPVLYITYGALRANRSPCPAGAGRSYYTPNCGAASGPPNPYSRGCSYITRCARV

>PpRALF2.1
RRLLAARRRSYISYGALNRNRSPCPARSGRSYYTPNCNSNAGPARPYTRGCLRITRCQRV

>PpRALF3.1
EQQLGFSPGAIYPDAHRVLAAAPNYYISYGSLNADRAPCPASSSGRSYYTTNCQSSGTPQPYVRSCSQITRCARG

>AmTrRALF1
RRSLYWRIVHYYISYGALSANRIPCPPRSGRSYYTHNCYKARGPVRPYHRGCNAITGCSRDVS

>AmTrRALF2
RRILADAENKYISYRALKKDSVPCSKAGVSYYNCGGTGPPNPYSRSCTTISGCARDTG

>AmTrRALF3
RRVVFELEQYISYGALMADRVPCPPGCGRPYYTKDCYRARGSVRPYDRGCSVITGCERYTG

>AmTrRALF4
RRLLGGAKRYISYGALKKDRVPCNHRGASYYNCKRTSRANPYRRGCSAITRCARILK

>AmTrRALF5
RRILATDQYISYNALKKNIVPCSKKGQSYYNCRAGGQANPYSRGCSAITRCARDTS

>AmTrRALF6
RRVLGAARQYLSYRALLKNRVPCNHRGNSYYNCKPRARANPYRRGCSIITRCARILK

>SmRALF1
SSSSPRDPLLGLLQAGGRSIQYYQEPPKKKQQHIHYISYGALSADRVPCAPMSGRSYYTPNCVAAKGPPDCYARRCSTITRCARDS

>SmRALF2
RRLLQQQQKQYISYGSLQANRVPCPPGSGRSYYTNNCNRATGAANPTQRGCSTITRCQ

>MpRALF1
RRILATKKKSNTKSSGYFISYSALSASRTSCPPRSGRSYYTRNCNSASGPVRPYSRGCSTISRCARDSG

>MpRALF2
PASPAAGGYYISYGALTADNVNCPPQSGRSYYTTNCNSASGPVRPYERTCSTITRCARDEV

>MpRALF3
RRMLAASGYYVGYGALTANRVPCPPQSGRSYYTPGCSTASGPVRPYTRGCSTITRCARDG

>AtRALF1
RRILATTKYISYQSLKRNSVPCSRRGASYYNCQNGAQANPYSRGCSKIARCRS

>AtRALF2
QKVIGYPAIGRDGARGCSPKDPSCPQQPEKPYKRGCEKITRCERDRRKQAHLRNPRKVLDVVAVMAKAKQLY

>AtRALF3
RKEIGYPKQRFGEDRTNPYEEITPPLIGGCDPKNPQTCLPKQPANPYRRGCLKITRCQRDV

>AtRALF4
RRYIGYDALKKNNVPCSRRGRSYYDCKKRRRNNPYRRGCSAITHCYRYAR

>AtRALF5
KRYIEYPPWQKHPCNPRFPTPDCYKRTPANPYRRGCTCISRCRRDCGGLSTWKKLLDTILKIPV

>AtRALF6
QTYINYNGMKGDIIPGCSSKNPKECVKIPAYSYNRGCEISTRCQRQQHSSSS

>AtRALF7
IKQINYKDLIKDTIPGCTSKNPKECVKVPANTYHRGCEISTRCHREQHSSSG

>AtRALF8
SVRYITYPAIDRGDHAVHCDKAHPNTCKKKQANPYRRGCGVLEGCHRETGPKPT

>AtRALF9
TRYITYPAIDRGDHAVHCDKAHPNTCKKKEANPYQRGCEKINRCRGG

>AtRALF10
VPVESRRKHLDYGVITKCAGPNPPPGCYPPGAQQKNPTPANEYRRGCSKITRCKRD

>AtRALF11
EPVESRNYIEYGAINKCAGPNPPPGCNPPGAEQKNPTPVNEYSRGCSKIHRCRRD

>AtRALF12
EPVESRNYIEYGAINKCAGPNPPPGCNPPGTEQKNPTPVNEYSRGCSKIHRCRRD

>AtRALF13
EPVESRNYIEYGAINKCAGPNPPPGCNPPGAEQKNPNPVNEYSRGCSKIHRCRRD

>AtRALF14
RRILQASRYISYEALKKNLPDNRRGEPDQRDNPYRRSCDVHSHCYRFTN

>AtRALF15
TRYISYRGMNHGDHAIHCDKAHPNTCKKQVANPYRRGCGTIERCRRDTGRK

>AtRALF16
RTLGYGSIKGDRIPACGYKNPNSCVKQPVNHYHRGCEKITRCARDAARYTESFNVDDDESPIINLH

>AtRALF17
NSIGAPAMREDLPKGCAPGSSAGCKMQPANPYKPGCEASQRCRGG

>AtRALF18
HRILQAKRFIDYEALKKNLPAKPDGKPDKPDNKYRRGCSAATGCYRFTN

>AtRALF19
RRQLAARRSYISYGALRKNNVPCSRRGRSYYDCKKRKRANPYRRGCSVITHCYRQTS

>AtRALF20
KTIGNPAMREDEPKGCPPGSPASCKMQPANPYKPGCEASQRCRGT

>AtRALF21
KVIGYPGLKPDLPCDHHRYPSACAPSEQPVNPYRRGCSKIHRCRRDSPPAPISRKMLIRGQLIYNNAYNAYIQYP

>AtRALF22
RRILAQKKYISYGAMRRNSVPCSRRGASYYNCQRGAQANPYSRGCSTITRCRR

>AtRALF23
RRILATRRYISYGALRRNTIPCSRRGASYYNCRRGAQANPYSRGCSAITRCRRS

>AtRALF24
RRVMMMRKQYISYETLRRDMVPCQKPGASYYACRSGQANAYNRGCSVITRCARDTNDIKT

>AtRALF25
NDNKRKYLLLDPCLRPNAPPGCHRQPYKPRTPVNVYSRGCTTINRCRRVQNP

>AtRALF26
RKGRKYLNPGVLDRCRGPNPPAGCHPHNSHHKPRVPVHNYSRGCSRITRCRRDA

>AtRALF27
RRILQQRKYLSYKTLQKQPTCDGRIAGNCIGTVNPKGATCTYYQRCKRAA

>AtRALF28
NEIGYPGMGRGDRQPGCDHGNCPPDQPANPYHRGCEKSKRCRGPDPPALPRKMI

>AtRALF29
KRYIEYPIRLDLGKGCDPRFPTAACYKRTPANPYRRPCTTANRCRRSTSSTRVPSLKTFVEIPPM

>AtRALF30
AGGGKFLNPGVLDPCLRPNPPPECQAPGSAGKPRERVNEYKVGCSKLTRCDRVG

>AtRALF31
RRVLMAQKRYIGYETLRRDMVPCQKPGASYYDCRSGQANSYSRGCDTITRCARDTNDINT

>AtRALF32
QRLTEEQAHKLSYGALRRNQPACDGGKRGESYSTQCLPPPSNPYSRGCSKHYRCGRDS

>AtRALF33
RRILATTKYISYGALRRNTVPCSRRGASYYNCRRGAQANPYSRGCSAITRCRR

>AtRALF34
RRSLYWRRTKYYISYGALSANRVPCPPRSGRSYYTHNCFRARGPVHPYSRGCSSITRCRR

>AtRALF35
RYIKYRAIAKDRVPDCTQDPKNCVRVPVNQYHLPPGCQNTTHCYREKYHI

>AtRALF36
DTINREQVISYESMRVNHAWGCSQKYPQFCQKTRANPYTKPPPKNSEAS

>AtRALF37
EDVISYEVLLQDHAWGCSPKFPRLSCLKQKANP
No tags attached.
Issue History
1969-12-31 17:33user614Statusnew => closed
1969-12-31 17:33user614Resolutionopen => fixed
2023-06-07 05:04guestNew Issue

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