MantisBT - MEGA
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0000066MEGA[All Projects] Feedbackpublic2016-02-23 13:522016-02-26 08:33
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gstecher 
normalminorhave not tried
resolvedno change required 
MEGA 11 (Graphical Interface version) 
 
Anita
Kuepper
akuepper@rams.colostate.edu
akuepper@rams.colostate.edu
0000066: Bootstrap SNP calls
Dear Sir or Madam,
I have recently used genotyping-by-sequencing to look at genetic relatedness between individuals. I would like to bootstrap the data now to get an idea how reliable the branches of my tree are. I have heard MEGA can provide this option, but I am running into problems when I try to read in the data because I do not have sequences, just SNP calls for many different loci for each individual.
Anita
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Issue History
2016-02-23 13:52guestNew Issue
2016-02-26 08:33gstecherNote Added: 0003614
2016-02-26 08:33gstecherStatusnew => resolved
2016-02-26 08:33gstecherResolutionopen => no change required
2016-02-26 08:33gstecherAssigned To => gstecher

Notes
(0003614)
gstecher   
2016-02-26 08:33   
Hi Anita,

I am writing in response to your question regarding the MEGA software. To use the bootstrap feature in MEGA for tree construction, you will need to use a multiple sequence alignment as input data. I am not sure you will be able to achieve what you describe with your data using MEGA.

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Best regards,

Glen Stecher