MantisBT - MEGA
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IDProjectCategoryView StatusDate SubmittedLast Update
0000740MEGA[All Projects] Feedbackpublic2018-04-18 04:002018-04-18 15:19
Reporterguest 
Assigned Togstecher 
PrioritynormalSeverityminorReproducibilityhave not tried
StatusresolvedResolutionno change required 
PlatformOSOS Version
Product VersionMEGA 11 (Graphical Interface version) 
Target VersionFixed in Version 
First NameJo
Last NameMayers
Emailjo.mayers@apha.gsi.gov.uk
Confirm Emailjo.mayers@apha.gsi.gov.uk
Summary0000740: Technical assistance required
DescriptionI need to generate a phylogenetic tree comparing the amino acid sequences between a panel of isolates and determining the number of AA substitutions . I believe I need to generate a maximum parsimony tree for this analysis, however, when I generate the tree in MEGA7 the number of changes are not shown. Could someone please provide assistance or recommend a suitable you tube video or tutorial?
Kind regards
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Date ModifiedUsernameFieldChange
2018-04-18 04:00guestNew Issue
2018-04-18 15:19gstecherNote Added: 0003984
2018-04-18 15:19gstecherStatusnew => resolved
2018-04-18 15:19gstecherResolutionopen => no change required
2018-04-18 15:19gstecherAssigned To => gstecher

Notes
(0003984)
gstecher   
2018-04-18 15:19   
Hi Jo,

I am writing in response to your recent question regarding the MEGA software. When generating a parsimony tree in MEGA, branch lengths are not automatically calculated. When the tree is shown, you should click View->Topology Only which will cause branch lengths to be calculated. If they are still not shown in the tree after this, click View->Show/Hide->Branch Lengths.

--
Best regards,

Glen Stecher
Institute for Genomics and Evolutionary Medicine
igem.temple.edu