MantisBT - MEGA
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0000775MEGACalculation Errorpublic2018-05-21 23:082018-05-30 13:14
guest 
gstecher 
normalminorhave not tried
resolvedno change required 
PCLinuxDebian
MEGA-CC 11 (command line version) 
 
Nidhi
Batra
nidhi.batra@igib.in
nidhi.batra@igib.in
0000775: error while running phylogeny or model generation
I am using MEGA 7 and was trying to generate a phylogenetic tree using maximum likelihood for > 1500 protein sequences. I ran the alignment also in MEGA using MUSCLE, it ran fine. While running either model selection program or phylogeny, i am getting the following error:

"insufficient data available for ML analysis. Please validate your sequence data"

I used the program earlier for 0000529:0000400 sequences and it ran fine. Does the number of sequences is an issue? Please help.
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Issue History
2018-05-21 23:08guestNew Issue
2018-05-22 09:48gstecherNote Deleted: 0000723
2018-05-22 09:48gstecherFile Deleted: Bug report_070627.txt
2018-05-30 13:14gstecherNote Added: 0004015
2018-05-30 13:14gstecherStatusnew => resolved
2018-05-30 13:14gstecherResolutionopen => no change required
2018-05-30 13:14gstecherAssigned To => gstecher

Notes
(0004015)
gstecher   
2018-05-30 13:14   
Hi Nidhi,

I am writing in response to your recent question regarding the MEGA software. The error message you describe seems to indicate that the handling of Gaps/Missing Data results in no data being available. In the Analysis Preferences dialog try setting the Gaps/Missing Data Treatment option to Use All Sites.

--
Best regards,

Glen Stecher
Institute for Genomics and Evolutionary Medicine
igem.temple.edu