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IDProjectCategoryView StatusDate SubmittedLast Update
0001932MEGA[All Projects] Feedbackpublic2020-04-20 08:212020-04-23 14:25
Reporterguest 
Assigned Togstecher 
PrioritynormalSeverityminorReproducibilityhave not tried
StatusresolvedResolutionno change required 
PlatformOS 
Product VersionMEGA 11 (Graphical Interface version) 
Target VersionFixed in Version 
Summary0001932: Placement of short reads on constrained topology
DescriptionI would like to add some short reads without changing the topology of the tree.

Is this possible to do within the MEGA X suite? I know that I could go the RAxML + pplacer way, but I have made the trees with MEGA and it would be more comfortable to keep working with the same software.

Thanks and best wishes,

Stefano
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First NameStefano
Last NameScilipoti
Emailss@bios.au.dk
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(0004451)
gstecher (administrator)
2020-04-23 14:25

Hi Stefano,

I am writing in response to your question regarding the MEGA software. I am not sure I understand what you are trying to do but it sounds like you might want to try the user tree analysis in MEGA. If you already have a tree in a newick file then from the main form you can run User Tree->Analyze User Tree by Maximum Likelihood where your two input data files will be the newick file and a multiple sequence alignment that has the short reads added. I am not sure if this is what you really want but it sounds like the closest thing available in MEGA.

--
Best regards,
Glen Stecher
Institute for Genomics and Evolutionary Medicine
igem.temple.edu

- Issue History
Date Modified Username Field Change
1969-12-31 17:33 user19 Status new => assigned
1969-12-31 17:33 user19 Assigned To => agasky
2020-04-20 08:21 guest New Issue
2020-04-23 14:25 gstecher Note Added: 0004451
2020-04-23 14:25 gstecher Status new => resolved
2020-04-23 14:25 gstecher Resolution open => no change required
2020-04-23 14:25 gstecher Assigned To => gstecher


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