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IDProjectCategoryView StatusDate SubmittedLast Update
0002083MEGA[All Projects] Feedbackpublic2020-06-19 10:502020-06-19 14:44
Reporterguest 
Assigned Togstecher 
PrioritynormalSeverityminorReproducibilityhave not tried
StatusresolvedResolutionwon't fix 
PlatformOS 
Product VersionMEGA 11 (Graphical Interface version) 
Target VersionFixed in Version 
Summary0002083: Problem regarding ML tree building
DescriptionHello Sir/Madam,
I Soumila Mondal, a research scholar from Banaras Hindu University is writing to you as I am facing difficulties during tree building. Currently, I am working on a protein that bears selenocysteine (U). During ML tree construction the software pop up a window having a message unusual amino acid residue found. The residue U is so important for my study thus I can't delete it. Thus I request you to give me suggestions on this matter as soon as possible.
Thanking You
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First NameSOUMILA
Last NameMONDAL
Emailsoumila.bhu@gmail.com
Confirm Emailsoumila.bhu@gmail.com
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-  Notes
(0004482)
gstecher (administrator)
2020-06-19 14:44

Hi Soumila,

I am writing in response to your question regarding the MEGA software. Unfortunately the models used in MEGA do not support selenocysteine so the only option for analyzing the data in MEGA would be to treat those bases as ambiguous data. I think you might want to look at RAxML, Phyml, PAML, or some other software for constructing an ML tree and see if any of them support selenocysteine.

--
Best regards,
Glen Stecher
Institute for Genomics and Evolutionary Medicine
igem.temple.edu

- Issue History
Date Modified Username Field Change
1969-12-31 17:33 user19 Status new => closed
1969-12-31 17:33 user19 Resolution open => fixed
2020-06-19 10:50 guest New Issue
2020-06-19 14:44 gstecher Note Added: 0004482
2020-06-19 14:44 gstecher Status new => resolved
2020-06-19 14:44 gstecher Resolution open => won't fix
2020-06-19 14:44 gstecher Assigned To => gstecher


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