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IDProjectCategoryView StatusDate SubmittedLast Update
0003725MEGA[All Projects] Feedbackpublic2022-06-01 00:522022-06-08 07:57
Reporterguest 
Assigned Togstecher 
PrioritynormalSeverityminorReproducibilityhave not tried
StatusresolvedResolutionno change required 
PlatformOS 
Product VersionMEGA 11 (Graphical Interface version) 
Target VersionFixed in Version 
Summary0003725: regarding error J amino acid
DescriptionHi MEGA!

I am encountering a problem. A aligned my protein pop gen data in Geneious software and got the haplotypes. When I exported it to MEGA, MEGA says there is an invalid element- J amino acid. However, this does not appear in any of my sequences using other programs or if I use the MEGA text editor.

What is the reason for this problem and how can I address this? Thanks
TagsNo tags attached.
Attach Tags (Separate by ",")
First NameBryan
Last NameSubong
Emailbjsubong@chem.s.u-tokyo.ac.jp
Confirm Emailbjsubong@chem.s.u-tokyo.ac.jp
Attached Files

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-  Notes
(0002586)
user614
1969-12-31 17:33

We should really try to improve this but it is not a high priority. However, maybe we can take a quick look at the code and make sure that there isn't some obvious way to make this more efficient.
(0005630)
gstecher (administrator)
2022-06-08 07:57

Hi Bryan,

Thanks for sending those files. MEGA does not support the ambiguous J symbol for leucine or isoleucine so you may need to replace those symbols with the ? symbol for missing data to analyze your data in MEGA. Regarding the second error, there are sequences of differing lengths and it seems the sequences have not been aligned. Below is a screen shot of the first file where a couple of sequences are shorter than the others.

- Issue History
Date Modified Username Field Change
1969-12-31 17:33 user614 Tag Attached: image_export
1969-12-31 17:33 user614 Note Added: 0002586
1969-12-31 17:33 user614 Category Tree Explorer => Image Export
1969-12-31 17:33 user614 Product Version 5.0 =>
2022-06-01 00:52 guest New Issue
2022-06-08 07:57 gstecher Note Added: 0005630
2022-06-08 07:57 gstecher Status new => resolved
2022-06-08 07:57 gstecher Resolution open => no change required
2022-06-08 07:57 gstecher Assigned To => gstecher


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