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ID | Project | Category | View Status | Date Submitted | Last Update | ||||
0001510 | MEGA | [All Projects] Feedback | public | 2019-10-24 08:57 | 2019-10-25 10:48 | ||||
Reporter | guest | ||||||||
Assigned To | gstecher | ||||||||
Priority | normal | Severity | minor | Reproducibility | have not tried | ||||
Status | resolved | Resolution | no change required | ||||||
Platform | OS | ||||||||
Product Version | MEGA 11 (Graphical Interface version) | ||||||||
Target Version | Fixed in Version | ||||||||
Summary | 0001510: Reverse Complement | ||||||||
Description | When adding a fasta file into mega, it sometimes switches the sequences to plus/plus and to know this for future alignments w/ other sequences, I need to BLAST it so I know if I need to change it to plus/minus to match other seqs in the alignment. Is there a way to add seq's from fasta files w/o having to blast each one each time added to find out if it needs to have "reverse complement" applied so that all sequences in the alignment are the same plus/minus? | ||||||||
Tags | No tags attached. | ||||||||
Attach Tags | (Separate by ",") | ||||||||
First Name | Connie | ||||||||
Last Name | Roderick | ||||||||
croderick@usgs.gov | |||||||||
Confirm Email | croderick@usgs.gov | ||||||||
Attached Files | |||||||||
Notes | |
(0004321) gstecher (administrator) 2019-10-25 10:48 |
Hi Connie, I am writing in response to your question regarding the MEGA software. MEGA does not automatically reverse complement any sequence data. You can test this by opening your fasta file in a text editor and comparing it to the display in MEGA. -- Best regards, Glen Stecher Institute for Genomics and Evolutionary Medicine igem.temple.edu |
Issue History | |||
Date Modified | Username | Field | Change |
2019-10-24 08:57 | guest | New Issue | |
2019-10-25 10:48 | gstecher | Note Added: 0004321 | |
2019-10-25 10:48 | gstecher | Status | new => resolved |
2019-10-25 10:48 | gstecher | Resolution | open => no change required |
2019-10-25 10:48 | gstecher | Assigned To | => gstecher |
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