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IDProjectCategoryView StatusDate SubmittedLast Update
0001585MEGA[All Projects] Feedbackpublic2019-11-21 10:312019-11-21 11:47
Reporterguest 
Assigned Togstecher 
PrioritynormalSeverityminorReproducibilityhave not tried
StatusresolvedResolutionno change required 
PlatformOS 
Product VersionMEGA 11 (Graphical Interface version) 
Target VersionFixed in Version 
Summary0001585: Multiple sequence input
DescriptionHi,
I'm trying to use MEGA to make a tree and an alignment, starting from a curated multi-fasta file containing a large number of sequences (>1000).
Each sequence in the unique text file is preceded by a header, for example >Acas_1_10
How can I input a this large number of sequences ? Would it be possible to create a single MEGA file containing all sequences ? Or do I have to create an individual file for each protein to be aligned?
Thanks in advance for your help!
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First NameFrancois
Last NameRobin
Emailfrancois.robin@upmc.fr
Confirm Emailfrancois.robin@upmc.fr
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-  Notes
(0004336)
gstecher (administrator)
2019-11-21 11:47

Hi Francois,

I am writing in response to your question regarding the MEGA software. Are you trying to concatenate multiple gene sequences to create a single alignment file? If so, there is an option in MEGAX to concatenate multiple fasta files. First, you copy all the files you want to concatenate into a single directory. Then on the MEGAX main form, click Data->Concatenate Sequence Alignments. MEGAX will prompt you for the directory containing your fasta files and then try and concatenate them.

--
Best regards,
Glen Stecher
Institute for Genomics and Evolutionary Medicine
igem.temple.edu

- Issue History
Date Modified Username Field Change
1969-12-31 17:33 ddview New Issue
2019-11-21 10:31 guest New Issue
2019-11-21 11:47 gstecher Note Added: 0004336
2019-11-21 11:47 gstecher Status new => resolved
2019-11-21 11:47 gstecher Resolution open => no change required
2019-11-21 11:47 gstecher Assigned To => gstecher


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