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IDProjectCategoryView StatusDate SubmittedLast Update
0001828MEGACalculation Errorpublic2020-03-08 10:542020-03-09 07:26
Reporterguest 
Assigned Togstecher 
PrioritynormalSeverityminorReproducibilityhave not tried
StatusresolvedResolutionno change required 
PlatformPCOSWindows 
Product VersionMEGA 11 (Graphical Interface version) 
Target VersionFixed in Version 
Summary0001828: treating missing data and gaps in multigene phylogenetics
DescriptionReport bugs or other issues regarding the MEGA software
I got a trouble to solve treating missing data/ gaps in multigene phylogenetic tree with Neighbor-joining tree. My data can use Maximum likelihood tree and Maximum parsimony tree test with only "use all sites" option, but I do not know which method MEGA program used to treat missing data and gaps. I only see the infomation about "Pairwise-Deletion" and "Complete-Deletion", not "use all sites" option. How is it different to other programs like RAxML and MrBayes? Thank you.
Additional InformationI also tested another published data from a paper "A multigene phylogeny toward a new phylogenetic classification of Leotiomycetes". and met similar problems.
TagsNo tags attached.
Attach Tags (Separate by ",")
First NameSon
Last NameHoang Kim
EmailHoangkimsonbio@gmail.com
Confirm EmailHoangkimsonbio@gmail.com
Attached Files? file icon 5 genes SSU LSU TEF RPB1 RPB2 2.fasta (388,514 bytes) 2020-03-08 10:54

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-  Notes
(0004418)
gstecher (administrator)
2020-03-09 07:26

Hi Son,

I am writing in response to your question regarding the MEGA software. Neighbor-Joining is a distance-based method so some options such as gap treatment are different than for Maximimum Likelhood and Maximium Parsimony analyses. The 'use all sites' option is not available for distance-based methods as it is not valid for the method.

--
Best regards,
Glen Stecher
Institute for Genomics and Evolutionary Medicine
igem.temple.edu

- Issue History
Date Modified Username Field Change
1969-12-31 17:33 user19 Note Added: 0001610
1969-12-31 17:33 user19 Status new => assigned
1969-12-31 17:33 user19 Assigned To => user19
1969-12-31 17:33 user19 Note Added: 0001684
1969-12-31 17:33 user19 Note Added: 0001685
1969-12-31 17:33 user19 Note Added: 0001686
1969-12-31 17:33 user19 Note Edited: 0001686
1969-12-31 17:33 user19 Status assigned => closed
1969-12-31 17:33 user19 Resolution open => fixed
2020-03-08 10:54 guest New Issue
2020-03-08 10:54 guest File Added: 5 genes SSU LSU TEF RPB1 RPB2 2.fasta
2020-03-09 07:25 gstecher Note Deleted: 0001610
2020-03-09 07:25 gstecher Note Deleted: 0001684
2020-03-09 07:25 gstecher Note Deleted: 0001685
2020-03-09 07:26 gstecher Note Deleted: 0001686
2020-03-09 07:26 gstecher Note Added: 0004418
2020-03-09 07:26 gstecher Status new => resolved
2020-03-09 07:26 gstecher Resolution open => no change required
2020-03-09 07:26 gstecher Assigned To => gstecher


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