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IDProjectCategoryView StatusDate SubmittedLast Update
0002003MEGA[All Projects] Feedbackpublic2020-05-22 11:572020-05-27 15:59
Reporterguest 
Assigned Togstecher 
PrioritynormalSeverityminorReproducibilityhave not tried
StatusresolvedResolutionno change required 
PlatformOS 
Product VersionMyPEG 
Target VersionFixed in Version 
Summary0002003: Error in phylogeny tree with bootstraps
DescriptionHello,

I have a question, when I ran NJ tree construction using bootstraping, I got the following error message:
"Notice: During the bootstrapping process 332 of 500 replicate failed and so bootstrap values are based on only 168 replicates. Appreciate your help.

Thanks,
Mahnaz

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First NameMahnaz
Last NameKiani
Emailmahnazk@thegreencell.com
Confirm Emailmahnazk@thegreencell.com
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-  Notes
(0001794)
user19
1969-12-31 17:33

Were just not implemented. I Implemented these for Mac/Linux.
(0004466)
gstecher (administrator)
2020-05-27 15:59

Hi Mahnaz,

I am writing in response to your question regarding the MEGA software. The warning message you received is not an error. It just indicates that the random sampling of data during the bootstrap procedure produced some data sets for which some distances could not be computed and so those replicates were tossed out. It indicates that the alignment is problematic for distance-based tree construction. You might want to check the quality of the data or use some other tree construction method such as Maximum Likelihood.

--
Best regards,
Glen Stecher
Institute for Genomics and Evolutionary Medicine
igem.temple.edu

- Issue History
Date Modified Username Field Change
1969-12-31 17:33 user19 Note Added: 0001794
1969-12-31 17:33 user19 Status new => resolved
1969-12-31 17:33 user19 Resolution open => fixed
1969-12-31 17:33 user19 Assigned To => user19
2020-05-22 11:57 guest New Issue
2020-05-27 15:59 gstecher Note Added: 0004466
2020-05-27 15:59 gstecher Status new => resolved
2020-05-27 15:59 gstecher Resolution open => no change required
2020-05-27 15:59 gstecher Assigned To => gstecher


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