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| ID | Project | Category | View Status | Date Submitted | Last Update | ||||
| 0003081 | MEGA | Tree Explorer | public | 2021-09-16 23:14 | 2021-09-20 08:22 | ||||
| Reporter | guest | ||||||||
| Assigned To | gstecher | ||||||||
| Priority | normal | Severity | minor | Reproducibility | have not tried | ||||
| Status | resolved | Resolution | no change required | ||||||
| Platform | Mac | OS | OS X | ||||||
| Product Version | |||||||||
| Target Version | Fixed in Version | ||||||||
| Summary | 0003081: Neighbour Joining tree not computed | ||||||||
| Description | Hi, I have been trying to make a Neighbour joining tree, from an aligned file (aligned with ClustalW) and keep getting the following error message: MEGA has encountered an error and cannot continue with this analysis: Some pairwise distances could not be estimated. For example, between sequences 38 and 26 I have managed to make a maximum likelihood tree but not a minimum evolution tree. Do you have any advice on how to fix this? | ||||||||
| Steps To Reproduce | Import file and generate Neighbour joining tree with default parameters | ||||||||
| Tags | No tags attached. | ||||||||
| Attach Tags | (Separate by ",") | ||||||||
| First Name | Ricarda | ||||||||
| Last Name | Streich | ||||||||
| ricarda.streich@kcl.ac.uk | |||||||||
| Confirm Email | ricarda.streich@kcl.ac.uk | ||||||||
| Attached Files |  clustalw (1).fasta (87,526 bytes) 2021-09-16 23:14 | ||||||||
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(0004712) gstecher (administrator) 2021-09-20 08:22 |
Hi Ricarda, I am writing in response to your question regarding the MEGA software. Distance-based methods require common sites for pairwise comparisons but some sequence pairs in your data have none. If you want to use distance-based methods with that data, you will need to remove sequences that have not common sites with other sequences. Best regards, Glen Stecher Institute for Genomics and Evolutionary Medicine igem.temple.edu |
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Issue History |
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| Date Modified | Username | Field | Change |
| 2021-09-16 23:14 | guest | New Issue | |
| 2021-09-16 23:14 | guest | File Added: clustalw (1).fasta | |
| 2021-09-20 08:22 | gstecher | Note Added: 0004712 | |
| 2021-09-20 08:22 | gstecher | Status | new => resolved |
| 2021-09-20 08:22 | gstecher | Resolution | open => no change required |
| 2021-09-20 08:22 | gstecher | Assigned To | => gstecher |
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Issue History |
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