0003685: Problem in distance calcuation

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0003685

MEGA

Distance Data Explorer

public

2022-05-16 07:39

2023-04-14 18:52

Reporter

guest

Assigned To

Priority

normal

Severity

minor

Reproducibility

have not tried

Status

new

Resolution

open

Platform

Windows

Product Version

MEGA 11 (Graphical Interface version)

Target Version

Fixed in Version

Summary

0003685: Problem in distance calcuation

Description

Dear Mega11 programmers,
I have an aligned set of sequences (see attached), for which I wanted to construct a NJ-tree in Mega11 (Build#: 11220201-x86_64) using Tamura 3-parameter model, but it failed.

I'd be curious, what could be the problem

Thanks for your help:

Balázs Vajna, PhD, assistant professor
Department of Microbiology
Eötvös Loránd University, Budapest, Hungary
Homepage: mikrobi.elte.hu/vajna_balazs_en

Steps To Reproduce

1, I've loaded the attached fasta file to Mega11 with "Analyze" option as nucleotid, non-protein coding sequence.
2, Then I started the Phylogeny / Construct/Test Neighbor-Joining Tree
3, I've set the following paramaters:
- Substitution model: Tamura 3-parameter model including transitions and transversions
- using uniform rates with homogenous patterns
- missing data: pairwise deletions
4, After starting the calculation I've received the following error message:

Distance calculation failed - distance matrix could not be computed. Error id is 4518 (Tamura-distance incalculable for some pairs.)

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First Name

Balázs

Last Name

Vajna

vajna.balazs@ttk.elte.hu

Confirm Email

vajna.balazs@ttk.elte.hu

Attached Files

Rizobium_atpD_probe_ARB_aligned.fas (7,931 bytes) 2022-05-16 07:39

Relationships

Notes
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Issue History
Date Modified	Username	Field	Change
2022-05-16 07:39	guest	New Issue
2022-05-16 07:39	guest	File Added: Rizobium_atpD_probe_ARB_aligned.fas
2023-04-14 18:52	guest	Note Added: 0006669

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