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IDProjectCategoryView StatusDate SubmittedLast Update
0001510MEGA[All Projects] Feedbackpublic2019-10-24 08:572019-10-25 10:48
Reporterguest 
Assigned Togstecher 
PrioritynormalSeverityminorReproducibilityhave not tried
StatusresolvedResolutionno change required 
PlatformOS 
Product VersionMEGA 11 (Graphical Interface version) 
Target VersionFixed in Version 
Summary0001510: Reverse Complement
DescriptionWhen adding a fasta file into mega, it sometimes switches the sequences to plus/plus and to know this for future alignments w/ other sequences, I need to BLAST it so I know if I need to change it to plus/minus to match other seqs in the alignment.

Is there a way to add seq's from fasta files w/o having to blast each one each time added to find out if it needs to have "reverse complement" applied so that all sequences in the alignment are the same plus/minus?

 
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First NameConnie
Last NameRoderick
Emailcroderick@usgs.gov
Confirm Emailcroderick@usgs.gov
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(0004321)
gstecher (administrator)
2019-10-25 10:48

Hi Connie,

I am writing in response to your question regarding the MEGA software. MEGA does not automatically reverse complement any sequence data. You can test this by opening your fasta file in a text editor and comparing it to the display in MEGA.

--
Best regards,
Glen Stecher
Institute for Genomics and Evolutionary Medicine
igem.temple.edu

- Issue History
Date Modified Username Field Change
2019-10-24 08:57 guest New Issue
2019-10-25 10:48 gstecher Note Added: 0004321
2019-10-25 10:48 gstecher Status new => resolved
2019-10-25 10:48 gstecher Resolution open => no change required
2019-10-25 10:48 gstecher Assigned To => gstecher


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