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| ID | Project | Category | View Status | Date Submitted | Last Update | ||||
| 0002748 | MEGA | Alignment Explorer | public | 2021-04-17 11:24 | 2021-04-20 11:06 | ||||
| Reporter | guest | ||||||||
| Assigned To | gstecher | ||||||||
| Priority | normal | Severity | minor | Reproducibility | have not tried | ||||
| Status | resolved | Resolution | no change required | ||||||
| Platform | PC | OS | Windows | ||||||
| Product Version | MEGA 11 (Graphical Interface version) | ||||||||
| Target Version | Fixed in Version | ||||||||
| Summary | 0002748: invalid base found: ? (in line 172) | ||||||||
| Description | In version 10.2.4 I wanted to do a Phylogenetic Analysis from the attached file to find the best fit DNA model. When cliclikg on the Phlylogenetic Analysis menu I get this error message: invalid base found: ? (in line 172). Can you help me please what causes this problem? | ||||||||
| Additional Information | I have chosen Invertebrate mitochondrion as genetic code. But the same happens with standard code too. | ||||||||
| Tags | No tags attached. | ||||||||
| Attach Tags | (Separate by ",") | ||||||||
| First Name | Janos | ||||||||
| Last Name | Agoston | ||||||||
| agoston.janos123@gmail.com | |||||||||
| Confirm Email | agoston.janos123@gmail.com | ||||||||
| Attached Files |  Cacopsylla_COI nt CW.mas (47,589 bytes) 2021-04-17 11:24 | ||||||||
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Notes |
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(0002166) Nikita Vikhrev (reporter) 1969-12-31 17:33 |
Hi Dan, Please ask the user to send the newick file causing the problem. Thanks. Koichiro |
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(0002174) user19 1969-12-31 17:33 |
Sent user email on 3/30 (the day bug was submitted) I have not yet heard back from them. Sent the user a second email asking for a response. |
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(0004576) gstecher (administrator) 2021-04-20 11:06 |
Hi Janos, I am writing in response to your question regarding the MEGA software. Somehow, in the alignment file you uploaded, two sequences have an odd character at the last position (they are colored grey in the Alignment Explorer - see screen shot below). If you delete those two bases, the analysis will work. |
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Notes |
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Issue History |
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| Date Modified | Username | Field | Change |
| 1969-12-31 17:33 | Nikita Vikhrev | Note Added: 0002166 | |
| 1969-12-31 17:33 | user19 | Note Added: 0002174 | |
| 1969-12-31 17:33 | user19 | Status | new => assigned |
| 1969-12-31 17:33 | user19 | Assigned To | => user19 |
| 2021-04-17 11:24 | guest | New Issue | |
| 2021-04-17 11:24 | guest | File Added: Cacopsylla_COI nt CW.mas | |
| 2021-04-20 11:06 | gstecher | Note Added: 0004576 | |
| 2021-04-20 11:06 | gstecher | Status | new => resolved |
| 2021-04-20 11:06 | gstecher | Resolution | open => no change required |
| 2021-04-20 11:06 | gstecher | Assigned To | => gstecher |
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Issue History |
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