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IDProjectCategoryView StatusDate SubmittedLast Update
0000585MEGACaption Expertpublic2017-11-01 00:282017-11-07 09:16
Reporterguest 
Assigned Togstecher 
PrioritynormalSeverityminorReproducibilityhave not tried
StatusresolvedResolutionfixed 
PlatformPCOSWindows 
Product VersionMEGA 11 (Graphical Interface version) 
Target VersionFixed in Version 
Summary0000585: Best-fit model select suggests values not allowed when constructing phylogenetic trees
DescriptionBest-fit substitution model suggests +I and +G values that cannot be entered under the "No of discrete gamma catagories" section during maximum-likelihood tree construction. Error message is "1.09 is not a valid integer value".
Steps To ReproduceRun Best-fit substitution model on data, +G values below 2 cause this error.
Click phylogeny, select "construct/test ML tree".
Set parameters - Test of phylogeny = bootstrap
replicates = 1000
substitution type = nucleotide
model/method = any
rates among sites = gamma

gaps/missing data = use all sites (data was trimmed to shortest sequence +-530bp).
all codon positions selected
ML Heuristic method = NNI
Initial tree for ML = automatic (default NJ/BioNJ)
Branch swap filter = Moderate
No of discrete gamma categories = 1.09
Initially entering gamma = 1.09 last and pressing enter allows the run to proceed. Trying to edit this value later for a different analysis results in error message.
TagsNo tags attached.
Attach Tags (Separate by ",")
First NameAlmero
Last NameOosthuizen
Emailalmero@nzg.ac.za
Confirm Emailalmero@nzg.ac.za
Attached Filespng file icon MEGA7 error.png (96,635 bytes) 2017-11-01 00:28

- Relationships

-  Notes
(0000481)
paul (reporter)
1969-12-31 17:33

Suggestion is saved. Sent thank you email to user. This suggestion is closed.
(0003881)
gstecher (administrator)
2017-11-07 09:16

Hi Almero,

I am writing in response to the bug report you recently submitted regarding the MEGA software. To resolve the error you can do the following:

    Close MEGA if it is opened
    Navigate to the AppData directory by entering %appdata% in the Windows Explorer address bar and pressing enter
    This will open the c:\users\username\AppData\Roaming directory. Once in that directory, delete the folder MEGA7_7170509-x86_64
    Open MEGA and run the analysis

On a side note, Gamma Parameter and No of Discrete Gamma Categories are completely unrelated, so you should not enter 1.09 for No of Discrete Categories (it should be an integer value, such as 5).

--
Best regards,

Glen Stecher
Institute for Genomics and Evolutionary Medicine
igem.temple.edu

- Issue History
Date Modified Username Field Change
2017-11-01 00:28 guest New Issue
2017-11-01 00:28 guest File Added: MEGA7 error.png
2017-11-07 09:16 gstecher Note Added: 0003881
2017-11-07 09:16 gstecher Status new => resolved
2017-11-07 09:16 gstecher Resolution open => fixed
2017-11-07 09:16 gstecher Assigned To => gstecher


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