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IDProjectCategoryView StatusDate SubmittedLast Update
0000727MEGA[All Projects] Feedbackpublic2018-04-08 16:112018-04-16 16:29
Reporterguest 
Assigned Togstecher 
PrioritynormalSeverityminorReproducibilityhave not tried
StatusresolvedResolutionnot fixable 
PlatformOS 
Product VersionMEGA 11 (Graphical Interface version) 
Target VersionFixed in Version 
Summary0000727: MEGA 7 crashes with inadequate RAM - wrong error
DescriptionHi,

1. We have a FASTA file with 3000 sequences (short peptides, proteins). When trying to align it using ClustalW or Muscle in MEGA 7, it crashed in both Mac and Windows with higher CPU/RAM.

2.Please advice how to submit a FASTA (unaligned) file to MEGA 7 CC via HPC cluster and use ClustalW or Muscle to align it and run other phylogenetic analysis using Linux? We noticed the pdf for MEGA 7 CC is not upto date with submitting input file and aligning it etc.

Thanks,
Diaz
TagsNo tags attached.
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First NameD
Last NameDiaz
Emaildildiaz.d@gmail.com
Confirm Emaildildiaz.d@gmail.com
Attached Files

- Relationships

-  Notes
(0000665)
paul (reporter)
1969-12-31 17:33

This is not a bug. User\'s file is not a standard Newick format that is why MEGA can not read it. Reply to user about his format. Close the report.
(0003979)
gstecher (administrator)
2018-04-16 16:29

Hi Dil,

MEGA does not handle the MUSCLE alignment on its own, but rather through a separate program. In order to use the MUSCLE alignment, you will need to download the separate MUSCLE application from this website: http://www.drive5.com/muscle/downloads.htm [^] and run the alignment using that. As you'll see there, there is only a 32-bit application available for Windows, and a 64-bit application for Mac OS or Linux. To run the MUSCLE alignment, please refer to the manual located on their website: http://www.drive5.com/muscle/manual/index.html [^] which includes these instructions:

PATH or pathname
The binary file must be in one of the directories specified in your PATH variable, or you must type the full path to the file, e.g. for Linux:

/usr/bin/muscle6.0.98_i86linux32 -in seqs.fa -out seqs.afa

Under the Windows CMD shell, path names are separated by backslashes, e.g.:

C:\MyPrograms\muscle6.2.98_win32.exe -in seqs.fa -out seqs.afa
 
Run muscle from a shell
muscle will not work if you try to start it in a graphical interface like Windows Explorer or the Mac Finder. You must start it from a command window or shell.
Please let me know if you have any questions.

Christina

- Issue History
Date Modified Username Field Change
2018-04-08 16:11 guest New Issue
2018-04-10 13:13 gstecher File Deleted: Bacteria.final.phy
2018-04-16 16:29 gstecher Note Added: 0003979
2018-04-16 16:29 gstecher Status new => resolved
2018-04-16 16:29 gstecher Resolution open => not fixable
2018-04-16 16:29 gstecher Assigned To => gstecher


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