0001239: ERROR MESSAGE: Error-Allignement Failed: MUSCLE Log file did not end properly, suggesting an unhandled exception

View Issue Details [ Jump to Notes ]

[ Issue History ] [ Print ]

Project

Category

View Status

Date Submitted

Last Update

0001239

MEGA

Alignment Explorer

public

2019-05-21 03:10

2019-05-21 08:58

Reporter

guest

Assigned To

gstecher

Priority

normal

Severity

minor

Reproducibility

have not tried

Status

resolved

Resolution

not fixable

Platform

Windows

Product Version

Target Version

Fixed in Version

Summary

0001239: ERROR MESSAGE: Error-Allignement Failed: MUSCLE Log file did not end properly, suggesting an unhandled exception

Description

Report bugs or other issues regarding the MEGA software
I'm using the MEGA X sofware downloaded, Windows 10
I am trying to align reference sequences and my sequences and also get phylogeny tree.
I get an error message
The error message is
Error-Allignement Failed: MUSCLE Log file did not end properly, suggesting an unhandled exception.

I have a attached the fasta file with the reads. I hope you can explain what is wrong as I have another file giving the same error.

Steps To Reproduce

Selected Align | Edit/Build Alignment from the launch bar.
Select Create a new alignment and then select DNA.
From the Alignment Explorer window, I selected Data | Open | Retrieve sequences from a
file and select the “k1-8.txt” file (attached).
On the Alignment Explorer main menu, I selected Edit | Select All.
On the Alignment Explorer i selected the icon that looks like a flexing arm.
And select Align DNA.

Additional Information

urgently help as I haveanother folder to work on too

Tags

No tags attached.

Attach Tags

(Separate by ",")

First Name

Irene

Last Name

Mutuku

mutukumirene@gmail.com

Confirm Email

mutukumirene@gmail.com

Attached Files

k1-k8_refgenomesfasta.txt (965,653 bytes) 2019-05-21 03:10

Relationships

Notes
(0004249) gstecher (administrator) 2019-05-21 08:58	Hi Irene, I am writing in response to the bug report you submitted regarding the MEGA software. The alignment fails with your data file because the MUSCLE program is not able to allocate enough memory (MUSCLE on Windows is a 32-bit application) to complete the analysis. To get around this, you can download and use 64-bit MUSCLE for macOS or Linux. Or you could use something like MAFFT which is designed to align larger data sets. -- Best regards, Glen Stecher Institute for Genomics and Evolutionary Medicine igem.temple.edu

Issue History
Date Modified	Username	Field	Change
2019-05-21 03:10	guest	New Issue
2019-05-21 03:10	guest	File Added: k1-k8_refgenomesfasta.txt
2019-05-21 08:58	gstecher	Note Added: 0004249
2019-05-21 08:58	gstecher	Status	new => resolved
2019-05-21 08:58	gstecher	Resolution	open => not fixable
2019-05-21 08:58	gstecher	Assigned To	=> gstecher