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IDProjectCategoryView StatusDate SubmittedLast Update
0001259MEGA[All Projects] Feedbackpublic2019-06-04 21:022019-06-07 13:09
Reporterguest 
Assigned Togstecher 
PrioritynormalSeverityminorReproducibilityhave not tried
StatusresolvedResolutionnot fixable 
PlatformOS 
Product VersionMEGA 11 (Graphical Interface version) 
Target VersionFixed in Version 
Summary0001259: Error Alignment Failed
DescriptionHi
I tried to run 134 whole genome DNA sequence (100kbp) alignment using MUSCLE (neighbor-joining). I keep getting this message "Error Alignment failed: Muscle logfile didn't end properly, suggesting an unhandled exception." However, If i tried 12 sequence on the same protein, the program will work fine. Is there any way I can solve this problem. Thank you for your time to read this email.

Kind Regards,
Heng Ku
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First Nameheng
Last NameKu
Emailkuheng3@gmail.com
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(0004257)
gstecher (administrator)
2019-06-07 13:09

Hi Heng,

I am writing in response to the bug report you recently submitted regarding the MEGA software. Unfortunately, MEGA is limited in the amount of data it can perform sequence alignment on. You want try MAFFT, which is more suitable for such a large data set.

--
Best regards,
Glen Stecher
Institute for Genomics and Evolutionary Medicine
igem.temple.edu

- Issue History
Date Modified Username Field Change
2019-06-04 21:02 guest New Issue
2019-06-07 13:09 gstecher Note Added: 0004257
2019-06-07 13:09 gstecher Status new => resolved
2019-06-07 13:09 gstecher Resolution open => not fixable
2019-06-07 13:09 gstecher Assigned To => gstecher


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