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IDProjectCategoryView StatusDate SubmittedLast Update
0002846MEGA[All Projects] Feedbackpublic2021-05-25 17:382021-05-27 07:27
Reporterguest 
Assigned Togstecher 
PrioritynormalSeverityminorReproducibilityhave not tried
StatusresolvedResolutionwon't fix 
PlatformOS 
Product VersionMEGA 11 (Graphical Interface version) 
Target VersionFixed in Version 
Summary0002846: muscle error
DescriptionHi
I am Dipon, student of university of british coloumbia. I am trying to use this software for alignment of DNA sequence. I have 2000 DNA sequence. Whenever I am trying to align these sequences using muscle the software showing me this error. " Error-Alignment failed: Muscle log file did not end properly, suggesting an unhandled exception." please let me know how to over come these problem.

Thanking you in advance and looking forward to hear from you
kind regards,
Dipon
TagsNo tags attached.
Attach Tags (Separate by ",")
First NameDipon
Last NameSaha
Emaildipon1992saha@student.ubc.ca
Confirm Emaildipon1992saha@student.ubc.ca
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-  Notes
(0004597)
gstecher (administrator)
2021-05-27 07:27

Hi Dipon,

I am writing in response to the bug report you recently submitted regarding the MEGA software. MUSCLE is actually a separate software that MEGA uses for sequence alignment. Because it is a separate software, we are not able to debug it and see why the alignment failed. Also, MUSCLE is not designed for use with very large alignments. You should probably use something like MAFFT for sequence alignment as it is engineered for large data.

Best regards,
Glen Stecher
Institute for Genomics and Evolutionary Medicine
igem.temple.edu

- Issue History
Date Modified Username Field Change
1969-12-31 17:33 user614 Note Added: 0002302
1969-12-31 17:33 user614 Status new => acknowledged
1969-12-31 17:33 user614 Status acknowledged => closed
2021-05-25 17:38 guest New Issue
2021-05-27 07:22 gstecher Note Deleted: 0002302
2021-05-27 07:22 gstecher File Deleted: crash-report.txt
2021-05-27 07:27 gstecher Note Added: 0004597
2021-05-27 07:27 gstecher Status new => resolved
2021-05-27 07:27 gstecher Resolution open => won't fix
2021-05-27 07:27 gstecher Assigned To => gstecher


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