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IDProjectCategoryView StatusDate SubmittedLast Update
0000031MEGA[All Projects] Suggestionpublic2016-02-05 06:442016-02-05 15:01
Reporterguest 
Assigned Togstecher 
PrioritynormalSeverityminorReproducibilityhave not tried
StatusassignedResolutionopen 
PlatformPCOSLinux 
Product VersionMEGA-CC 11 (command line version) 
Target VersionFixed in Version 
Summary0000031: Genetic codes
DescriptionHi

I am using the command line version on some mitochondrial sequences and I want to test for selection using Z-test but I have a problem because I can't change the genetic code on megaproto, and I am getting stop codons in my sequences. Is there a way to manipulate the file or the .mao file so it can change to mitochondrial genetic code?
Thanks in advance
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First NameThemis
Last NameGiannoulis
Emailthemisgia@hotmail.com
Confirm Emailthemisgia@hotmail.com
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-  Notes
(0003590)
gstecher (administrator)
2016-02-05 15:01

Hi Themis,

I am writing in response to the question you recently posted to the MEGA bug tracker regarding how to set the genetic code table for the z-test of selection analysis. Thanks for the feedback. We will fix the bug you found soon. In the meantime, I think you can work around the problem as follows:

    Add a couple of lines for the genetic code table to the .mao file as in the attached example.
    If you need a different code table from the one in the example then:
        open megaproto
        select coding data
        select some other analysis (like Phylogeny->Construct/Test Maximum Likelihood Tree...)
        set the Substitution Type to Amino Acid
        select the desired genetic code table
        save that mao file
        open that mao file in a text editor and copy the two code table lines from that file
        paste the two code table lines into your .mao file for the z-test

--
Best regards,

Glen Stecher

- Issue History
Date Modified Username Field Change
2016-02-05 06:44 guest New Issue
2016-02-05 15:01 gstecher Assigned To => gstecher
2016-02-05 15:01 gstecher Status new => assigned
2016-02-05 15:01 gstecher Note Added: 0003590


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